nebnext ultra ii dna library preparation kit  (New England Biolabs)

Article Title: The P323L substitution in the SARS-CoV-2 polymerase (NSP12) confers a selective advantage during infection
Article Snippet: .. The amplicon products were then used in Illumina NEBNext Ultra II DNA Library preparation. ..

Article Title: mRNA-Engineered CRISPR-Cas epigenetic editors enable durable and efficient gene silencing in Vivo
Article Snippet: .. Amplicons were generated using KOD DNA Polymerase (TOYOBO) and 379 pooled for each condition so that each sample contained all associated amplicons and was used 380 as input for NEBNext Ultra II DNA library preparation, and amplified with unique dual indexes to 381 allow for multiplexing. ..

Article Title: mRNA-engineered CRISPR-Cas epigenetic editors enable durable and efficient gene silencing in vivo
Article Snippet: .. Amplicons were generated using KOD DNA Polymerase (TOYOBO), pooled for each condition so that each sample contained all associated amplicons, used as input for NEBNext Ultra II DNA library preparation, and amplified with unique dual indexes to allow for multiplexing. ..

Article Title: The P323L substitution in the SARS-CoV-2 polymerase (NSP12) confers a selective advantage during infection.
Article Snippet: .. The amplicon products were then used in Illumina NEBNext Ultra II DNA Library preparation. ..

Article Title: The P323L substitution in the SARS-CoV-2 polymerase (NSP12) confers a selective advantage during infection
Article Snippet: .. The amplicon products were then used in Illumina NEBNext Ultra II DNA Library preparation. ..

Article Title: A novel NGS-compatible Enzymatic Strategy Enables Carryover Contamination Removal and Enhances Sequencing Performance
Article Snippet: For this, genomic DNA was sheared to 250bp (20ng/ul DNA, 1X TE 50ul, Covaris AFA minitubes (Covaris PN 500514), 10% intensity, 5 cycles, 200 Burst, 40seconds). .. Using 100ng of sheared gDNA per sample, NEBNext Ultra II DNA Library Preparation (NEB #E7645) reagents were used for end preparation repair and adaptor ligation following manufacturer recommendations. ..

Article Title: mRNA-Engineered CRISPR-Cas epigenetic editors enable durable and efficient gene silencing in Vivo
Article Snippet: .. Amplicons were generated using KOD DNA Polymerase (TOYOBO) and 379 pooled for each condition so that each sample contained all associated amplicons and was used 380 as input for NEBNext Ultra II DNA library preparation, and amplified with unique dual indexes to 381 allow for multiplexing. ..

Article Title: mRNA-engineered CRISPR-Cas epigenetic editors enable durable and efficient gene silencing in vivo
Article Snippet: .. Amplicons were generated using KOD DNA Polymerase (TOYOBO), pooled for each condition so that each sample contained all associated amplicons, used as input for NEBNext Ultra II DNA library preparation, and amplified with unique dual indexes to allow for multiplexing. ..

Article Title: HM-DyadCap - capture and mapping of 5-hydroxymethylcytosine/5-methylcytosine CpG dyads in mammalian DNA.
Article Snippet: .. Subsequently, DNA as purified using the Oligo Clean and Concentrator kit Zymo Research) following the manufacturer’s protocol. nd repair and adapter ligation ne microgram of fragmented DNA, either glucosylated or on-glucosylated, was subjected to end repair and adapter ligtion using the NEBNext Ultra II DNA Library Preparation it (NEB, E7103; version 6.1_5/20). .. DNA purification and ize selection was carried out using NEBNext Sample Purifiation Beads (NEB, E7103) according to the protocol with ead volumes corresponding to a target fragment size of 200 p. Elution was carried out according to the manufacturer’s rotocol, and DNA concentration was determined with the uantus Fluorometer (Promega).

Article Title: The P323L substitution in the SARS-CoV-2 polymerase (NSP12) confers a selective advantage during infection.
Article Snippet: .. The amplicon products were then used in Illumina NEBNext Ultra II DNA Library preparation. ..

Article Title: Metagenomic Insights Into the Role of Gut Microbes in the Defensive Ink “Tsunabi” of Physeteroid Whales
Article Snippet: .. A metagenomic library with an insert size of 300 bp was constructed from high‐quality DNA extracted from each sample using the NEBNext Ultra II DNA Library Preparation (New England Biolabs, Ipswich, MA, USA), NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set) (New England Biolabs), and AMPure XP Beads (Beckman Coulter Inc., Brea, CA, USA) following the manufacturer's manual instructions. .. The Agilent 2200 TapeStation system (Agilent Technologies, Santa Clara, USA) was used to determine the fragment size distribution.

Article Title: A Vibrio cholerae viral satellite maximizes its spread and inhibits phage by remodeling hijacked phage coat proteins into small capsids
Article Snippet: gene, ( Vibrio cholerae ) Assembly: GCF 007050395. 1 , orf17; tcaP (PLE5_2017) , N/A , Locus tag: DM782 RS18270 Protein accession: WP 00191219 5.1 , satellite external scaffolding protein. .. Commercial kit , NEBNext Ultra II DNA Library preparation , New England Biolabs , NEB #E7645, E7103 , . ..

Article Title: A novel NGS-compatible Enzymatic Strategy Enables Carryover Contamination Removal and Enhances Sequencing Performance
Article Snippet: For this, genomic DNA was sheared to 250bp (20ng/ul DNA, 1X TE 50ul, Covaris AFA minitubes (Covaris PN 500514), 10% intensity, 5 cycles, 200 Burst, 40seconds). .. Using 100ng of sheared gDNA per sample, NEBNext Ultra II DNA Library Preparation (NEB #E7645) reagents were used for end preparation repair and adaptor ligation following manufacturer recommendations. ..

Article Title: mRNA-Engineered CRISPR-Cas epigenetic editors enable durable and efficient gene silencing in Vivo
Article Snippet: .. Amplicons were generated using KOD DNA Polymerase (TOYOBO) and 379 pooled for each condition so that each sample contained all associated amplicons and was used 380 as input for NEBNext Ultra II DNA library preparation, and amplified with unique dual indexes to 381 allow for multiplexing. ..

Article Title: mRNA-engineered CRISPR-Cas epigenetic editors enable durable and efficient gene silencing in vivo
Article Snippet: .. Amplicons were generated using KOD DNA Polymerase (TOYOBO), pooled for each condition so that each sample contained all associated amplicons, used as input for NEBNext Ultra II DNA library preparation, and amplified with unique dual indexes to allow for multiplexing. ..

Article Title: mRNA-Engineered CRISPR-Cas epigenetic editors enable durable and efficient gene silencing in Vivo
Article Snippet: .. Amplicons were generated using KOD DNA Polymerase (TOYOBO) and 379 pooled for each condition so that each sample contained all associated amplicons and was used 380 as input for NEBNext Ultra II DNA library preparation, and amplified with unique dual indexes to 381 allow for multiplexing. ..

Article Title: mRNA-engineered CRISPR-Cas epigenetic editors enable durable and efficient gene silencing in vivo
Article Snippet: .. Amplicons were generated using KOD DNA Polymerase (TOYOBO), pooled for each condition so that each sample contained all associated amplicons, used as input for NEBNext Ultra II DNA library preparation, and amplified with unique dual indexes to allow for multiplexing. ..

Article Title: HM-DyadCap - capture and mapping of 5-hydroxymethylcytosine/5-methylcytosine CpG dyads in mammalian DNA.
Article Snippet: .. Subsequently, DNA as purified using the Oligo Clean and Concentrator kit Zymo Research) following the manufacturer’s protocol. nd repair and adapter ligation ne microgram of fragmented DNA, either glucosylated or on-glucosylated, was subjected to end repair and adapter ligtion using the NEBNext Ultra II DNA Library Preparation it (NEB, E7103; version 6.1_5/20). .. DNA purification and ize selection was carried out using NEBNext Sample Purifiation Beads (NEB, E7103) according to the protocol with ead volumes corresponding to a target fragment size of 200 p. Elution was carried out according to the manufacturer’s rotocol, and DNA concentration was determined with the uantus Fluorometer (Promega).

Article Title: HM-DyadCap - capture and mapping of 5-hydroxymethylcytosine/5-methylcytosine CpG dyads in mammalian DNA.
Article Snippet: .. Subsequently, DNA as purified using the Oligo Clean and Concentrator kit Zymo Research) following the manufacturer’s protocol. nd repair and adapter ligation ne microgram of fragmented DNA, either glucosylated or on-glucosylated, was subjected to end repair and adapter ligtion using the NEBNext Ultra II DNA Library Preparation it (NEB, E7103; version 6.1_5/20). .. DNA purification and ize selection was carried out using NEBNext Sample Purifiation Beads (NEB, E7103) according to the protocol with ead volumes corresponding to a target fragment size of 200 p. Elution was carried out according to the manufacturer’s rotocol, and DNA concentration was determined with the uantus Fluorometer (Promega).

Article Title: Metagenomic Insights Into the Role of Gut Microbes in the Defensive Ink “Tsunabi” of Physeteroid Whales
Article Snippet: .. A metagenomic library with an insert size of 300 bp was constructed from high‐quality DNA extracted from each sample using the NEBNext Ultra II DNA Library Preparation (New England Biolabs, Ipswich, MA, USA), NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set) (New England Biolabs), and AMPure XP Beads (Beckman Coulter Inc., Brea, CA, USA) following the manufacturer's manual instructions. .. The Agilent 2200 TapeStation system (Agilent Technologies, Santa Clara, USA) was used to determine the fragment size distribution.

Article Title: Metagenomic Insights Into the Role of Gut Microbes in the Defensive Ink “Tsunabi” of Physeteroid Whales
Article Snippet: .. A metagenomic library with an insert size of 300 bp was constructed from high‐quality DNA extracted from each sample using the NEBNext Ultra II DNA Library Preparation (New England Biolabs, Ipswich, MA, USA), NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set) (New England Biolabs), and AMPure XP Beads (Beckman Coulter Inc., Brea, CA, USA) following the manufacturer's manual instructions. .. The Agilent 2200 TapeStation system (Agilent Technologies, Santa Clara, USA) was used to determine the fragment size distribution.